› 7b0p

X-ray diffraction
1.94Å resolution

In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus in space group P21212

Released:
DOI: 10.2210/pdb7b0p/pdb
PDB entry 7b0p coloured by chain, front view.
PDB entry 7b0p coloured by chain, side view.
PDB entry 7b0p coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis.
Olatunji S, Bowen K, Huang CY, Weichert D, Singh W, Tikhonova IG, Scanlan EM, Olieric V, Caffrey M
Nat Commun 12 4254 (2021)
PMID: 34253723

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypothetical Membrane Spanning Protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.64 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q813T3 (Residues: 1-218; Coverage: 100%)
Gene name: BC_1526
Sequence domains: Protein of unknown function (DUF1461)

Ligands and Environments

3 bound ligands:
Ligand OLC 12 x OLC
Ligand GOL 15 x GOL
Ligand PG5 1 x PG5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21212
Unit cell:
a: 51.166Å b: 192.235Å c: 76.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.246
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Effects of extracellular vesicles derived from oral bacteria on osteoclast differentiation and activation.
Kim et al. (2022)
3 more

1 mention without citation

Bacterial Lipoprotein Posttranslational Modifications. New Insights and Opportunities for Antibiotic and Vaccine Development.
Smithers et al. (2021)