› 7avr

X-ray diffraction
2Å resolution

The tetrameric structure of haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2

Released:
DOI: 10.2210/pdb7avr/pdb
PDB entry 7avr coloured by chain, front view.
PDB entry 7avr coloured by chain, side view.
PDB entry 7avr coloured by chain, top view.
Source organism: Paraglaciecola agarilytica NO2
Primary publication:
The tetrameric structure of the novel haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2.
Mazur A, Prudnikova T, Grinkevich P, Mesters JR, Mrazova D, Chaloupkova R, Damborsky J, Kuty M, Kolenko P, Kuta Smatanova I
Acta Crystallogr D Struct Biol 77 347-356 (2021)
PMID: 33645538

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 307 amino acids
Theoretical weight: 34.53 KDa
Source organism: Paraglaciecola agarilytica NO2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K6XNL5 (Residues: 1-1, 3-301; Coverage: 100%)
Gene names: GAGA_3124, dhmA1
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand CL 18 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 117.385Å b: 155.506Å c: 155.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Mini Review: Advances in 2-Haloacid Dehalogenases.
Wang et al. (2021)
2 other articles