X-ray diffraction
2.9Å resolution

Crystal structure of S-nitrosylated nitrosoglutathione reductase(GSNOR)from Chlamydomonas reinhardtii, in complex with NAD+


Function and Biology Details

Reaction catalysed:
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
S-(hydroxymethyl)glutathione dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 378 amino acids
Theoretical weight: 40.48 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
  • Canonical: A0A2K3D6R4 (Residues: 1-378; Coverage: 100%)
Gene name: CHLRE_12g543400v5
Sequence domains:

Ligands and Environments

Cofactor: Ligand NAD 6 x NAD
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 78.386Å b: 143.294Å c: 206.271Å
α: 90° β: 90° γ: 90°
R R work R free
0.184 0.18 0.257
Expression system: Escherichia coli