X-ray diffraction
1.7Å resolution

Pseudomonas stutzeri nitrous oxide reductase mutant, H583N

Source organism: Pseudomonas stutzeri
Entry authors: Zhang L, Bill E, Kroneck PMH, Einsle O

Function and Biology Details

Reaction catalysed:
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.93 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P19573 (Residues: 1-638; Coverage: 100%)
Gene name: nosZ
Sequence domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 69.289Å b: 76.452Å c: 109.485Å
α: 90° β: 93.38° γ: 90°
R R work R free
0.163 0.161 0.199
Expression system: Escherichia coli BL21(DE3)