7apy

X-ray diffraction
1.78Å resolution

Pseudomonas stutzeri nitrous oxide reductase mutant, D576A

Released:

Function and Biology Details

Reaction catalysed:
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.91 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19573 (Residues: 1-638; Coverage: 100%)
Gene name: nosZ
Sequence domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 68.964Å b: 76.627Å c: 108.563Å
α: 90° β: 93.41° γ: 90°
R-values:
R R work R free
0.169 0.166 0.225
Expression system: Escherichia coli BL21(DE3)