› 7ao7

X-ray diffraction
2.55Å resolution

Structure of CYP153A from Polaromonas sp. in complex with octan-1-ol

Released:
DOI: 10.2210/pdb7ao7/pdb
PDB entry 7ao7 coloured by chain, front view.
PDB entry 7ao7 coloured by chain, side view.
PDB entry 7ao7 coloured by chain, top view.
Source organism: Polaromonas sp.
Primary publication:
Substrate Anchoring and Flexibility Reduction in CYP153AM.aq Leads to Highly Improved Efficiency toward Octanoic Acid
Rapp LR, Marques SM, Zukic E, Rowlinson B, Sharma M, Grogan G, Damborsky J, Hauer B
ACS Catalysis 11 3182-3189 (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 418 amino acids
Theoretical weight: 47.54 KDa
Source organism: Polaromonas sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q11ZY2 (Residues: 1-418; Coverage: 100%)
Gene name: Bpro_5301
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 6 x HEM
Ligand OC9 5 x OC9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 72.503Å b: 116.194Å c: 288.309Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.254
Expression system: Escherichia coli BL21(DE3)

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