› 7ant

X-ray diffraction
1.52Å resolution

Structure of CYP153A from Polaromonas sp.

Released:
DOI: 10.2210/pdb7ant/pdb
PDB 7ant coloured by chain and viewed from the front.
PDB 7ant coloured by chain and viewed from the side.
PDB 7ant coloured by chain and viewed from the top.
Source organism: Polaromonas sp. JS666
Primary publication:
Substrate Anchoring and Flexibility Reduction in CYP153AM.aq Leads to Highly Improved Efficiency toward Octanoic Acid
Rapp LR, Marques SM, Zukic E, Rowlinson B, Sharma M, Grogan G, Damborsky J, Hauer B
ACS Catalysis 11 3182-3189 (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 47.54 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q11ZY2 (Residues: 1-418; Coverage: 100%)
Gene name: Bpro_5301
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEC 2 x HEC
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 72.852Å b: 98.501Å c: 112.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.231
Expression system: Escherichia coli BL21(DE3)

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