X-ray diffraction
1.66Å resolution

MlghC, GDP-mannoheptose C4 reductase from Campylobacter jejuni with NADP bound

Source organism: Campylobacter jejuni
Entry authors: Naismith JH, Woodward L

Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
GDP-L-fucose synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 39.3 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
  • Canonical: Q0P8I6 (Residues: 1-344; Coverage: 99%)
Gene names: Cj1428c, fcl
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments

Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 57.8Å b: 131.96Å c: 59.33Å
α: 90° β: 105.84° γ: 90°
R R work R free
0.174 0.173 0.202
Expression system: Escherichia coli