› 7al9

X-ray diffraction
1.74Å resolution

human GTP cyclohydrolase I feedback regulatory protein (GFRP) in complex with phenylalanine

Released:
DOI: 10.2210/pdb7al9/pdb
PDB entry 7al9 coloured by chain, front view.
PDB entry 7al9 coloured by chain, side view.
PDB entry 7al9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A hybrid approach reveals the allosteric regulation of GTP cyclohydrolase I.
Ebenhoch R, Prinz S, Kaltwasser S, Mills DJ, Meinecke R, Rübbelke M, Reinert D, Bauer M, Weixler L, Zeeb M, Vonck J, Nar H
Proc Natl Acad Sci U S A 117 31838-31849 (2020)
PMID: 33229582

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP cyclohydrolase 1 feedback regulatory protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 87 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30047 (Residues: 1-84; Coverage: 100%)
Gene names: GCHFR, GFRP
Sequence domains: GTP cyclohydrolase I feedback regulatory protein (GFRP)

Ligands and Environments

2 bound ligands:
Ligand K 10 x K
Ligand PHE 10 x PHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 66.939Å b: 98.123Å c: 67.455Å
α: 90° β: 102.53° γ: 90°
R-values:
R R work R free
0.204 0.203 0.235
Expression system: Escherichia coli

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Citations

1 review citation

Tetrahydrobiopterin in Cell Function and Death Mechanisms.
Vasquez-Vivar et al. (2022)
3 other articles