› 7ahq

Electron Microscopy
3.6Å resolution

Cryo-EM structure of F-actin stabilized by trans-optoJASP-8

Released:
DOI: 10.2210/pdb7ahq/pdb
PDB entry 7ahq coloured by chain, front view.
PDB entry 7ahq coloured by chain, side view.
PDB entry 7ahq coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Cryo-EM Resolves Molecular Recognition Of An Optojasp Photoswitch Bound To Actin Filaments In Both Switch States.
Pospich S, Küllmer F, Nasufović V, Funk J, Belyy A, Bieling P, Arndt HD, Raunser S
Angew Chem Int Ed Engl 60 8678-8682 (2021)
PMID: 33449370
Related structures: EMD-11790

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

4 bound ligands:
Ligand ADP 5 x ADP
Ligand PO4 5 x PO4
Ligand MG 5 x MG
Ligand RLZ 5 x RLZ
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-11790

Quick links

Citations

7 citation in other articles

Structural basis of actin filament assembly and aging.
Oosterheert et al. (2022)
6 more

1 mention without citation

Cryo-EM Resolves Molecular Recognition Of An Optojasp Photoswitch Bound To Actin Filaments In Both Switch States.
Pospich et al. (2021)