7agt

X-ray diffraction
2.9Å resolution

Structure of the S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis acylated with Malonyl-coenzyme A

Released:

Function and Biology Details

Reaction catalysed:
A long-chain acyl-[mycocerosate synthase] + 4 methylmalonyl-CoA + 8 NADPH = a tetramethylated-mycocerosoyl-[mycocerosate synthase] + 4 CoA + 4 CO(2) + 8 NADP(+) + 4 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycocerosic acid synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 46.53 KDa
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02251 (Residues: 447-871; Coverage: 20%)
Gene names: BQ2027_MB2965C, mas
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 148.476Å b: 156.119Å c: 115.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.209
Expression system: Escherichia coli