› 7aea

Electron Microscopy
3.4Å resolution

Cryo-EM structure of human RNA Polymerase III elongation complex 2

Released:
DOI: 10.2210/pdb7aea/pdb
PDB entry 7aea coloured by chain, front view.
PDB entry 7aea coloured by chain, side view.
PDB entry 7aea coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
Girbig M, Misiaszek AD, Vorländer MK, Lafita A, Grötsch H, Baudin F, Bateman A, Müller CW
Nat Struct Mol Biol 28 210-219 (2021)
PMID: 33558764
Related structures: EMD-11742

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121062 (preferred)
Entry contents:
17 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (20 distinct):
DNA-directed RNA polymerase III subunit RPC1 Chain: A
Molecule details ›
Chain: A
Length: 1390 amino acids
Theoretical weight: 155.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14802 (Residues: 1-1390; Coverage: 100%)
Gene name: POLR3A
Sequence domains:
DNA-directed RNA polymerase III subunit RPC2 Chain: B
Molecule details ›
Chain: B
Length: 1133 amino acids
Theoretical weight: 127.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NW08 (Residues: 1-1133; Coverage: 100%)
Gene name: POLR3B
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: C
Molecule details ›
Chain: C
Length: 346 amino acids
Theoretical weight: 39.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15160 (Residues: 1-346; Coverage: 100%)
Gene names: POLR1C, POLR1E
Sequence domains:
DNA-directed RNA polymerase III subunit RPC9 Chain: D
Molecule details ›
Chain: D
Length: 148 amino acids
Theoretical weight: 16.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75575 (Residues: 1-148; Coverage: 100%)
Gene name: CRCP
Sequence domains: RNA polymerase Rpb4
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
Molecule details ›
Chain: E
Length: 210 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19388 (Residues: 1-210; Coverage: 100%)
Gene name: POLR2E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61218 (Residues: 1-127; Coverage: 100%)
Gene names: POLR2F, POLRF
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase III subunit RPC8 Chain: G
Molecule details ›
Chain: G
Length: 204 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y535 (Residues: 1-204; Coverage: 100%)
Gene names: KIAA1665, POLR3H, RPC8
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52434 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase III subunit RPC10 Chain: I
Molecule details ›
Chain: I
Length: 108 amino acids
Theoretical weight: 12.35 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y2Y1 (Residues: 1-108; Coverage: 100%)
Gene names: My010, POLR3K, RPC11
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
Molecule details ›
Chain: J
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62875 (Residues: 1-67; Coverage: 100%)
Gene name: POLR2L
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: K
Molecule details ›
Chain: K
Length: 133 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DPB6 (Residues: 1-133; Coverage: 100%)
Gene name: POLR1D
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Molecule details ›
Chain: L
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P53803 (Residues: 1-58; Coverage: 100%)
Gene name: POLR2K
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
DNA-directed RNA polymerase III subunit RPC5 Chain: M
Molecule details ›
Chain: M
Length: 708 amino acids
Theoretical weight: 80 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVU0 (Residues: 1-708; Coverage: 100%)
Gene names: KIAA1452, POLR3E
Sequence domains:
DNA-directed RNA polymerase III subunit RPC4 Chain: N
Molecule details ›
Chain: N
Length: 398 amino acids
Theoretical weight: 44.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P05423 (Residues: 1-398; Coverage: 100%)
Gene names: BN51, BN51T, POLR3D
Sequence domains: RNA polymerase III RPC4
DNA-directed RNA polymerase III subunit RPC3 Chain: O
Molecule details ›
Chain: O
Length: 534 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BUI4 (Residues: 1-534; Coverage: 100%)
Gene name: POLR3C
Sequence domains:
DNA-directed RNA polymerase III subunit RPC6 Chain: P
Molecule details ›
Chain: P
Length: 316 amino acids
Theoretical weight: 35.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H1D9 (Residues: 1-316; Coverage: 100%)
Gene name: POLR3F
Sequence domains: RNA polymerase Rpc34 subunit
DNA-directed RNA polymerase III subunit RPC7 Chain: Q
Molecule details ›
Chain: Q
Length: 223 amino acids
Theoretical weight: 25.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15318 (Residues: 1-223; Coverage: 100%)
Gene name: POLR3G
Sequence domains: DNA-directed RNA polymerase III subunit Rpc31
Non-template DNA Chain: S
Molecule details ›
Chain: S
Length: 46 nucleotides
Theoretical weight: 14.1 KDa
Template DNA Chain: T
Molecule details ›
Chain: T
Length: 46 nucleotides
Theoretical weight: 14.25 KDa
RNA Chain: R
Molecule details ›
Chain: R
Length: 19 nucleotides
Theoretical weight: 6.08 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 7 x ZN
Ligand SF4 1 x SF4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-11742

Quick links

Citations

10 review citations

RNA Polymerase III Subunit Mutations in Genetic Diseases.
Lata et al. (2021)
9 more

1 mention without citation

Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
Girbig et al. (2021)