7ady

X-ray diffraction
1.05Å resolution

CO-removed state of the active site of vanadium nitrogenase VFe protein

Released:
Source organism: Azotobacter vinelandii
Entry authors: Rohde M, Grunau K, Einsle O

Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nitrogenase vanadium-iron protein alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 474 amino acids
Theoretical weight: 53.95 KDa
Source organism: Azotobacter vinelandii
Expression system: Azotobacter vinelandii
UniProt:
  • Canonical: P16855 (Residues: 1-474; Coverage: 100%)
Gene name: vnfD
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Nitrogenase vanadium-iron protein beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 475 amino acids
Theoretical weight: 52.84 KDa
Source organism: Azotobacter vinelandii
Expression system: Azotobacter vinelandii
UniProt:
  • Canonical: P16856 (Residues: 1-475; Coverage: 100%)
Gene name: vnfK
Sequence domains: Nitrogenase component 1 type Oxidoreductase
Nitrogenase vanadium-iron protein delta chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 113 amino acids
Theoretical weight: 13.39 KDa
Source organism: Azotobacter vinelandii
Expression system: Azotobacter vinelandii
UniProt:
  • Canonical: P16857 (Residues: 1-113; Coverage: 100%)
Gene name: vnfG
Sequence domains: Vanadium/alternative nitrogenase delta subunit

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 75.576Å b: 80.027Å c: 107.2Å
α: 83.99° β: 72.48° γ: 75.03°
R-values:
R R work R free
0.121 0.121 0.141
Expression system: Azotobacter vinelandii