› 7acg

X-ray diffraction
1.85Å resolution

In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 9.8 monoacylglycerol

Released:
DOI: 10.2210/pdb7acg/pdb
PDB entry 7acg coloured by chain, front view.
PDB entry 7acg coloured by chain, side view.
PDB entry 7acg coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
9.8 MAG. A new host lipid for in meso (lipid cubic phase) crystallization of integral membrane proteins
van Dalsen L, Smithers L, Boland C, Weichert D, Caffrey M
Cryst Growth Des (2020)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate production protein AlgE Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 53.5 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18895 (Residues: 33-490; Coverage: 100%)
Gene names: PA3544, alg76, algE
Sequence domains: Alginate export

Ligands and Environments

7 bound ligands:
Ligand LH9 10 x LH9
Ligand 1PE 1 x 1PE
Ligand SO4 1 x SO4
Ligand C8E 1 x C8E
Ligand CIT 1 x CIT
Ligand PG0 1 x PG0
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 56.751Å b: 74.187Å c: 114.992Å
α: 90° β: 102.732° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli BL21(DE3)

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