› 7ab7

X-ray diffraction
1.8Å resolution

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) I52T Q148K variant

Released:
DOI: 10.2210/pdb7ab7/pdb
PDB entry 7ab7 coloured by chain, front view.
PDB entry 7ab7 coloured by chain, side view.
PDB entry 7ab7 coloured by chain, top view.
Source organism: Chloroflexus aggregans DSM 9485
Primary publication:
The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.
Röllen K, Granzin J, Remeeva A, Davari MD, Gensch T, Nazarenko VV, Kovalev K, Bogorodskiy A, Borshchevskiy V, Hemmer S, Schwaneberg U, Gordeliy V, Jaeger KE, Batra-Safferling R, Gushchin I, Krauss U
J Biol Chem 296 100662
PMID: 33862085

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Chloroflexus aggregans DSM 9485
Expression system: Escherichia coli
UniProt:
  • Canonical: B8GAY9 (Residues: 47-153; Coverage: 12%)
Gene name: Cagg_3753
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21212
Unit cell:
a: 53.083Å b: 111.992Å c: 39.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.195
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives.
Ohlendorf et al. (2022)
5 other articles

1 mention without citation

The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.
Röllen et al. (2021)