› 7a8b

X-ray diffraction
1.55Å resolution

rsGreen0.7-K206A-F145S partially in the green-on state

Released:
DOI: 10.2210/pdb7a8b/pdb
PDB 7a8b coloured by chain and viewed from the front.
PDB 7a8b coloured by chain and viewed from the side.
PDB 7a8b coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*.
De Zitter E, Hugelier S, Duwé S, Vandenberg W, Tebo AG, Van Meervelt L, Dedecker P
Angew Chem Int Ed Engl 60 10073-10081 (2021)
PMID: 33543524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.55 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A059PIQ0 (Residues: 3-238; Coverage: 94%)
Gene name: gfp
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R32
Unit cell:
a: 139.548Å b: 139.548Å c: 72.864Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.232
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification.
Bierbuesse et al. (2022)