› 7a86

X-ray diffraction
1.9Å resolution

rsGreen0.7-K206A-F145L partially in the green-off state

Released:
DOI: 10.2210/pdb7a86/pdb
PDB entry 7a86 coloured by chain, front view.
PDB entry 7a86 coloured by chain, side view.
PDB entry 7a86 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*.
De Zitter E, Hugelier S, Duwé S, Vandenberg W, Tebo AG, Van Meervelt L, Dedecker P
Angew Chem Int Ed Engl 60 10073-10081 (2021)
PMID: 33543524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 30.58 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A059PIQ0 (Residues: 3-238; Coverage: 94%)
Gene name: gfp
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand PGE 1 x PGE
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 48.102Å b: 58.29Å c: 58.466Å
α: 64.06° β: 71.06° γ: 72.61°
R-values:
R R work R free
0.183 0.181 0.21
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation.
Valenta et al. (2021)
2 other articles