› 7a80

X-ray diffraction
2.05Å resolution

rsGreen0.7-K206A-E222V in the green-on state

Released:
DOI: 10.2210/pdb7a80/pdb
PDB entry 7a80 coloured by chain, front view.
PDB entry 7a80 coloured by chain, side view.
PDB entry 7a80 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*.
De Zitter E, Hugelier S, Duwé S, Vandenberg W, Tebo AG, Van Meervelt L, Dedecker P
Angew Chem Int Ed Engl 60 10073-10081 (2021)
PMID: 33543524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 270 amino acids
Theoretical weight: 30.58 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A059PIQ0 (Residues: 3-238; Coverage: 94%)
Gene name: gfp
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NO3 4 x NO3
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 69.822Å b: 61.8Å c: 112.121Å
α: 90° β: 91.01° γ: 90°
R-values:
R R work R free
0.185 0.183 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation.
Valenta et al. (2021)
2 other articles