Electron Microscopy
3.2Å resolution

Nanodisc reconstituted human ABCB1 in complex with MRK16 Fab and vincristine


Function and Biology Details

Reactions catalysed:
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent translocase ABCB1 Chain: A
Molecule details ›
Chain: A
Length: 1280 amino acids
Theoretical weight: 141.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
  • Canonical: P08183 (Residues: 1-1280; Coverage: 100%)
Gene names: ABCB1, MDR1, PGY1
MRK16 Fab-fragment light chain Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 24.14 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
MRK16 Fab-fragment heavy chain Chain: C
Molecule details ›
Chain: C
Length: 218 amino acids
Theoretical weight: 23.42 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-11667
Expression system: Homo sapiens