› 7a5q

X-ray diffraction
1.68Å resolution

Crystal structure of VcSiaP bound to sialic acid

Released:
DOI: 10.2210/pdb7a5q/pdb
PDB entry 7a5q coloured by chain, front view.
PDB entry 7a5q coloured by chain, side view.
PDB entry 7a5q coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Triggering Closure of a Sialic Acid TRAP Transporter Substrate Binding Protein through Binding of Natural or Artificial Substrates.
Peter MF, Gebhardt C, Glaenzer J, Schneberger N, de Boer M, Thomas GH, Cordes T, Hagelueken G
J Mol Biol 433 166756 (2021)
PMID: 33316271

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic acid-binding periplasmic protein SiaP Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 33.79 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KR64 (Residues: 23-321; Coverage: 100%)
Gene names: VC_1779, siaP
Sequence domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

4 bound ligands:
Ligand SLB 2 x SLB
Ligand GOL 23 x GOL
Ligand PGE 2 x PGE
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 48.354Å b: 106.738Å c: 134.506Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.247
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Selective Nutrient Transport in Bacteria: Multicomponent Transporter Systems Reign Supreme.
Davies et al. (2021)
3 other articles

1 mention without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)