› 7a3p

X-ray diffraction
3.19Å resolution

Crystal structure of dengue 3 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10

Released:
DOI: 10.2210/pdb7a3p/pdb
PDB 7a3p coloured by chain and viewed from the front.
PDB 7a3p coloured by chain and viewed from the side.
PDB 7a3p coloured by chain and viewed from the top.
Source organisms:
Primary publication:
The epitope arrangement on flavivirus particles contributes to Mab C10's extraordinary neutralization breadth across Zika and dengue viruses.
Sharma A, Zhang X, Dejnirattisai W, Dai X, Gong D, Wongwiwat W, Duquerroy S, Rouvinski A, Vaney MC, Guardado-Calvo P, Haouz A, England P, Sun R, Zhou ZH, Mongkolsapaya J, Screaton GR, Rey FA
Cell 184 6052-6066.e18 (2021)
PMID: 34852239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope protein E Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 46.82 KDa
Source organism: Dengue virus 3
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q07019 (Residues: 167-559; Coverage: 60%)
Sequence domains:
Single Chain Variable Fragment Chains: H, I
Molecule details ›
Chains: H, I
Length: 144 amino acids
Theoretical weight: 15.68 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
Single Chain Variable Fragment Chains: L, M
Molecule details ›
Chains: L, M
Length: 154 amino acids
Theoretical weight: 15.63 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 53.744Å b: 101.412Å c: 249.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.284
Expression system: Drosophila melanogaster

Quick links

Citations

1 citations in other articles

Omicron, the great escape artist.
Flemming A. (2022)