X-ray diffraction
1.5Å resolution

Crystal structure of the c-Src SH3 domain mutant V111L-N113S-T114S in 7 M urea

Source organism: Gallus gallus
Entry authors: Camara-Artigas A, Plaza-Garrido M, Salinas-Garcia MC

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.72 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00523 (Residues: 82-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 28.49Å b: 40.532Å c: 43.613Å
α: 90° β: 104.66° γ: 90°
R R work R free
0.155 0.154 0.177
Expression system: Escherichia coli BL21(DE3)