› 7a29

Electron Microscopy
2.94Å resolution

Cryo-EM structure of the SARS-CoV-2 spike protein bound to neutralizing sybodies (Sb23) 2-up conformation

Released:
DOI: 10.2210/pdb7a29/pdb
PDB 7a29 coloured by chain and viewed from the front.
PDB 7a29 coloured by chain and viewed from the side.
PDB 7a29 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.
Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer MA, Gruzinov AY, Jeffries CM, Graewert MA, Svergun DI, Dobrev N, Remans K, Seeger MA, McInerney GM, Murrell B, Hällberg BM, Löw C
Nat Commun 11 5588 (2020)
PMID: 33149112
Related structures: EMD-11617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1288 amino acids
Theoretical weight: 142.4 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
Neutralising sybody (Sb23) Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 114 amino acids
Theoretical weight: 12.53 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 45 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.94Å
Relevant EMDB volumes: EMD-11617
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

2 review citations

Perspective on therapeutic and diagnostic potential of camel nanobodies for coronavirus disease-19 (COVID-19).
Bessalah et al. (2021)
1 more