7a08

Electron Microscopy
3.11Å resolution

CryoEM Structure of cGAS Nucleosome complex

Released:

Function and Biology Details

Reaction catalysed:
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Cyclic GMP-AMP synthase Chain: a
Molecule details ›
Chain: a
Length: 370 amino acids
Theoretical weight: 43.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8C6L5 (Residues: 139-507; Coverage: 73%)
Gene names: Cgas, Mb21d1
Sequence domains:
Histone H2A type 1-C Chains: b, f
Molecule details ›
Chains: b, f
Length: 129 amino acids
Theoretical weight: 14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93077 (Residues: 2-130; Coverage: 99%)
Gene names: H2AC6, H2AFL, HIST1H2AC
Sequence domains:
Histone H2B type 1-C/E/F/G/I Chains: c, g
Molecule details ›
Chains: c, g
Length: 125 amino acids
Theoretical weight: 13.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62807 (Residues: 2-126; Coverage: 99%)
Gene names: H2BC10, H2BC4, H2BC6, H2BC7, H2BC8, H2BFA, H2BFG, H2BFH, H2BFK, H2BFL, HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H3.3 Chains: d, h
Molecule details ›
Chains: d, h
Length: 135 amino acids
Theoretical weight: 15.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84243 (Residues: 2-136; Coverage: 99%)
Gene names: H3-3A, H3-3B, H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781
Histone H4 Chains: e, i
Molecule details ›
Chains: e, i
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 2-103; Coverage: 99%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Nucleosomal DNA strand 1 Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.15 KDa
Nucleosomal DNA strand 2 Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.6 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.11Å
Relevant EMDB volumes: EMD-11601
Expression system: Escherichia coli