› 7x2c

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the fenoldopam-bound D1 dopamine receptor and mini-Gs complex

Released:
DOI: 10.2210/pdb7x2c/pdb
PDB entry 7x2c coloured by chain, front view.
PDB entry 7x2c coloured by chain, side view.
PDB entry 7x2c coloured by chain, top view.
Source organisms:
Primary publication:
Ligand recognition and biased agonism of the D1 dopamine receptor.
Teng X, Chen S, Nie Y, Xiao P, Yu X, Shao Z, Zheng S
Nat Commun 13 3186 (2022)
PMID: 35676276
Related structures: EMD-32964

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-210832 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
D(1A) dopamine receptor Chain: F
Molecule details ›
Chain: F
Length: 473 amino acids
Theoretical weight: 52.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21728 (Residues: 1-446; Coverage: 100%)
Gene name: DRD1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 6-394; Coverage: 63%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: E
Molecule details ›
Chain: E
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CLR 1 x CLR
Ligand G3C 2 x G3C
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-32964
Expression systems:
  • Homo sapiens
  • Trichoplusia ni
  • Not provided

Quick links

Citations

10 citation in other articles

2-(Fluoromethoxy)-4'-(S-methanesulfonimidoyl)-1,1'-biphenyl (UCM-1306), an Orally Bioavailable Positive Allosteric Modulator of the Human Dopamine D1 Receptor for Parkinson's Disease.
García-Cárceles et al. (2022)
9 more