› 7x1u

Electron Microscopy
3.19Å resolution

Structure of Thyrotropin-Releasing Hormone Receptor bound with an Endogenous Peptide Agonist TRH.

Released:
DOI: 10.2210/pdb7x1u/pdb
PDB entry 7x1u coloured by chain, front view.
PDB entry 7x1u coloured by chain, side view.
PDB entry 7x1u coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into thyrotropin-releasing hormone receptor activation by an endogenous peptide agonist or its orally administered analogue.
Yang F, Zhang H, Meng X, Li Y, Zhou Y, Ling S, Sun D, Lv P, Liu L, Shi P, Tian C
Cell Res (2022)
PMID: 35352031
Related structures: EMD-32950

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225609 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Thyrotropin-releasing hormone receptor Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 45.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34981 (Residues: 1-398; Coverage: 100%)
Gene name: TRHR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
mini-G alpha q prtoein Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
ScFv16 Chain: D
Molecule details ›
Chain: D
Length: 251 amino acids
Theoretical weight: 26.68 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
Endogenous Peptide Agonist TRH Chain: E
Molecule details ›
Chain: E
Length: 4 amino acids
Theoretical weight: 362 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-32950
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

1 review citation

Biochemical and physiological insights into TRH receptor-mediated signaling.
Trubacova et al. (2022)
2 other articles

1 mention without citation

Non-Canonical Amino Acids as Building Blocks for Peptidomimetics: Structure, Function, and Applications.
Castro et al. (2023)