› 7w0o

Electron Microscopy
3.78Å resolution

Cryo-EM structure of a monomeric GPCR-Gi complex with peptide

Released:
DOI: 10.2210/pdb7w0o/pdb
PDB entry 7w0o coloured by chain, front view.
PDB entry 7w0o coloured by chain, side view.
PDB entry 7w0o coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into apelin receptor-G protein stoichiometry.
Yue Y, Liu L, Wu LJ, Wu Y, Wang L, Li F, Liu J, Han GW, Chen B, Lin X, Brouillette RL, Breault É, Longpré JM, Shi S, Lei H, Sarret P, Stevens RC, Hanson MA, Xu F
Nat Struct Mol Biol 29 688-697 (2022)
PMID: 35817871
Related structures: EMD-32246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225124 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 40.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Apelin receptor early endogenous ligand Chain: D
Molecule details ›
Chain: D
Length: 32 amino acids
Theoretical weight: 3.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P0DMC3 (Residues: 23-54; Coverage: 100%)
Gene names: APELA, ELA, TDL
Apelin receptor; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 477 amino acids
Theoretical weight: 54.31 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P35414 (Residues: 2-330; Coverage: 87%)
Gene names: AGTRL1, APJ, APLNR, cybC
Sequence domains:
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 297 amino acids
Theoretical weight: 31.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.78Å
Relevant EMDB volumes: EMD-32246
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

2 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
1 more