› 7ul5

Electron Microscopy
3.1Å resolution

CryoEM Structure of Inactive SSTR2 bound to Nb6

Released:
DOI: 10.2210/pdb7ul5/pdb
PDB entry 7ul5 coloured by chain, front view.
PDB entry 7ul5 coloured by chain, side view.
PDB entry 7ul5 coloured by chain, top view.
Source organisms:
Primary publication:
Structure determination of inactive-state GPCRs with a universal nanobody.
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022)
PMID: 36396979
Related structures: EMD-26592

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225579 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kappa-type opioid receptor; Somatostatin receptor type 2 Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30874 (Residues: 1-237, 254-369; Coverage: 96%)
  • Canonical: P41145 (Residues: 256-271; Coverage: 4%)
Gene names: OPRK, OPRK1, SSTR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody 6 Chain: D
Molecule details ›
Chain: D
Length: 133 amino acids
Theoretical weight: 14.73 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-26592
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

6 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
5 more

4 mentions without citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
3 more