7t94

Electron Microscopy
3.16Å resolution

Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225091 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Muscarinic acetylcholine receptor M2 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08172 (Residues: 4-232, 368-466; Coverage: 70%)
Gene name: CHRM2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(o) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P09471 (Residues: 1-354; Coverage: 100%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment Chain: E
Molecule details ›
Chain: E
Length: 256 amino acids
Theoretical weight: 27.34 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.16Å
Relevant EMDB volumes: EMD-25751
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda