› 7q2x

Electron Microscopy
3Å resolution

Cryo-EM structure of clamped S.cerevisiae condensin-DNA complex (Form I)

Released:
DOI: 10.2210/pdb7q2x/pdb
PDB entry 7q2x coloured by chain, front view.
PDB entry 7q2x coloured by chain, side view.
PDB entry 7q2x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Clamping of DNA shuts the condensin neck gate.
Lee BG, Rhodes J, Löwe J
Proc Natl Acad Sci U S A 119 e2120006119 (2022)
PMID: 35349345
Related structures: EMD-13783

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-121841 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Structural maintenance of chromosomes protein 2 Chain: A
Molecule details ›
Chain: A
Length: 1170 amino acids
Theoretical weight: 134.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38989 (Residues: 1-1170; Coverage: 100%)
Gene names: SMC2, YFR031C
Sequence domains:
Structural maintenance of chromosomes protein 4 Chain: B
Molecule details ›
Chain: B
Length: 1418 amino acids
Theoretical weight: 162.44 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae W303
UniProt:
  • Canonical: Q12267 (Residues: 1-1418; Coverage: 100%)
Gene names: L9449.5, SMC4, YLR086W
Sequence domains:
Condensin complex subunit 2 Chain: C
Molecule details ›
Chain: C
Length: 754 amino acids
Theoretical weight: 86.32 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae W303
UniProt:
  • Canonical: P38170 (Residues: 1-754; Coverage: 100%)
Gene names: BRN1, YBL0830, YBL097W
Sequence domains: Condensin complex subunit 2
Condensin complex subunit 1 Chain: D
Molecule details ›
Chain: D
Length: 1176 amino acids
Theoretical weight: 133.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae W303
UniProt:
  • Canonical: Q06156 (Residues: 1-1176; Coverage: 100%)
Gene names: L8479.14, LOC7, YCS4, YLR272C
Sequence domains:
DNA (36-MER) Chain: F
Molecule details ›
Chain: F
Length: 36 nucleotides
Theoretical weight: 11.23 KDa
DNA (36-MER) Chain: G
Molecule details ›
Chain: G
Length: 36 nucleotides
Theoretical weight: 10.91 KDa

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand BEF 2 x BEF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-13783
Expression systems:
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae W303

Quick links

Citations

1 review citation

Structural biology of SMC complexes across the tree of life.
Bürmann et al. (2023)
9 other articles