PDB 7o80 structure summary ‹ Protein Data Bank in Europe (PDBe)

Function and Biology Details

Reactions catalysed:

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

ATP + H(2)O = ADP + phosphate

Biochemical function:

not assigned

Biological process:

not assigned

Cellular component:

not assigned

Sequence domains:

Structure analysis Details

Assembly composition:

hetero 88-mer (preferred)

Assembly name:

SARS-CoV-2 NSP10-NSP16 2'-O-methyltransferase complex and RNA (preferred)

PDBe Complex ID:

PDB-CPX-243874 (preferred)

Entry contents:

81 distinct polypeptide molecules
7 distinct RNA molecules

Macromolecules (88 distinct):

Small ribosomal subunit protein eS27 Chain: AA

Molecule details ›

Chain: AA
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS28 Chain: AB

Molecule details ›

Chain: AB
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus

Ubiquitin-ribosomal protein eS31 fusion protein Chain: AC

Molecule details ›

Chain: AC
Length: 156 amino acids
Theoretical weight: 18 KDa
Source organism: Oryctolagus cuniculus

Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: AD

Molecule details ›

Chain: AD
Length: 133 amino acids
Theoretical weight: 14.5 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eS26 Chain: AE

Molecule details ›

Chain: AE
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein RACK1 Chain: AF

Molecule details ›

Chain: AF
Length: 317 amino acids
Theoretical weight: 35.12 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS14 Chain: AG

Molecule details ›

Chain: AG
Length: 56 amino acids
Theoretical weight: 6.69 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS2 Chain: AZ

Molecule details ›

Chain: AZ
Length: 295 amino acids
Theoretical weight: 32.97 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS1 Chain: Aa

Molecule details ›

Chain: Aa
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein uS5 Chain: Ab

Molecule details ›

Chain: Ab
Length: 293 amino acids
Theoretical weight: 31.29 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS3 Chain: Ac

Molecule details ›

Chain: Ac
Length: 281 amino acids
Theoretical weight: 31.15 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eS4 Chain: Ad

Molecule details ›

Chain: Ad
Length: 263 amino acids
Theoretical weight: 29.65 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS7 Chain: Ae

Molecule details ›

Chain: Ae
Length: 204 amino acids
Theoretical weight: 22.91 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS6 Chain: Af

Molecule details ›

Chain: Af
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS7 Chain: Ag

Molecule details ›

Chain: Ag
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS8 Chain: Ah

Molecule details ›

Chain: Ah
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS4 Chain: Ai

Molecule details ›

Chain: Ai
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS10 Chain: Aj

Molecule details ›

Chain: Aj
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS17 Chain: Ak

Molecule details ›

Chain: Ak
Length: 158 amino acids
Theoretical weight: 18.47 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS12 Chain: Al

Molecule details ›

Chain: Al
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS15 Chain: Am

Molecule details ›

Chain: Am
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS11 Chain: An

Molecule details ›

Chain: An
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS19 Chain: Ao

Molecule details ›

Chain: Ao
Length: 145 amino acids
Theoretical weight: 17.05 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS9 Chain: Ap

Molecule details ›

Chain: Ap
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS17 Chain: Aq

Molecule details ›

Chain: Aq
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS13 Chain: Ar

Molecule details ›

Chain: Ar
Length: 152 amino acids
Theoretical weight: 17.8 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eS19 Chain: As

Molecule details ›

Chain: As
Length: 145 amino acids
Theoretical weight: 16.1 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS10 Chain: At

Molecule details ›

Chain: At
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eS21 Chain: Au

Molecule details ›

Chain: Au
Length: 83 amino acids
Theoretical weight: 9.18 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS8 Chain: Av

Molecule details ›

Chain: Av
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein uS12 Chain: Aw

Molecule details ›

Chain: Aw
Length: 143 amino acids
Theoretical weight: 15.86 KDa
Source organism: Oryctolagus cuniculus

40S ribosomal protein S24 Chain: Ax

Molecule details ›

Chain: Ax
Length: 130 amino acids
Theoretical weight: 15.11 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS25 Chain: Ay

Molecule details ›

Chain: Ay
Length: 124 amino acids
Theoretical weight: 13.65 KDa
Source organism: Oryctolagus cuniculus

Small ribosomal subunit protein eS32 Chain: Az

Molecule details ›

Chain: Az
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein uL2 Chain: BA

Molecule details ›

Chain: BA
Length: 257 amino acids
Theoretical weight: 28.1 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL3 Chain: BB

Molecule details ›

Chain: BB
Length: 403 amino acids
Theoretical weight: 46.12 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL4 Chain: BC

Molecule details ›

Chain: BC
Length: 413 amino acids
Theoretical weight: 46.43 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL18 Chain: BD

Molecule details ›

Chain: BD
Length: 297 amino acids
Theoretical weight: 34.52 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL6 Chain: BE

Molecule details ›

Chain: BE
Length: 291 amino acids
Theoretical weight: 33.03 KDa
Source organism: Oryctolagus cuniculus

Ribosomal Protein uL30 Chain: BF

Molecule details ›

Chain: BF
Length: 247 amino acids
Theoretical weight: 29.2 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eL8 Chain: BG

Molecule details ›

Chain: BG
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL6 Chain: BH

Molecule details ›

Chain: BH
Length: 192 amino acids
Theoretical weight: 21.87 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL16 Chain: BI

Molecule details ›

Chain: BI
Length: 214 amino acids
Theoretical weight: 24.64 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL5 Chain: BJ

Molecule details ›

Chain: BJ
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Oryctolagus cuniculus

RNA-directed RNA polymerase nsp12 Chain: BK

Molecule details ›

Chain: BK
Length: 1071 amino acids
Theoretical weight: 121.57 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Oryctolagus cuniculus

Ribosomal protein eL13 Chain: BL

Molecule details ›

Chain: BL
Length: 211 amino acids
Theoretical weight: 24.33 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL14 Chain: BM

Molecule details ›

Chain: BM
Length: 218 amino acids
Theoretical weight: 23.87 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL15 Chain: BN

Molecule details ›

Chain: BN
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein L13a Chain: BO

Molecule details ›

Chain: BO
Length: 203 amino acids
Theoretical weight: 23.53 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL22 Chain: BP

Molecule details ›

Chain: BP
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein L18 Chain: BQ

Molecule details ›

Chain: BQ
Length: 188 amino acids
Theoretical weight: 21.7 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL19 Chain: BR

Molecule details ›

Chain: BR
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein L18a Chain: BS

Molecule details ›

Chain: BS
Length: 176 amino acids
Theoretical weight: 20.75 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL21 Chain: BT

Molecule details ›

Chain: BT
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eL22 Chain: BU

Molecule details ›

Chain: BU
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL14 Chain: BV

Molecule details ›

Chain: BV
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL24 Chain: BW

Molecule details ›

Chain: BW
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL23 Chain: BX

Molecule details ›

Chain: BX
Length: 156 amino acids
Theoretical weight: 17.77 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL24 Chain: BY

Molecule details ›

Chain: BY
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL27 Chain: BZ

Molecule details ›

Chain: BZ
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL15 Chain: Ba

Molecule details ›

Chain: Ba
Length: 148 amino acids
Theoretical weight: 16.64 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL29 Chain: Bb

Molecule details ›

Chain: Bb
Length: 245 amino acids
Theoretical weight: 26.72 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL30 Chain: Bc

Molecule details ›

Chain: Bc
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL31 Chain: Bd

Molecule details ›

Chain: Bd
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL32 Chain: Be

Molecule details ›

Chain: Be
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL33 Chain: Bf

Molecule details ›

Chain: Bf
Length: 110 amino acids
Theoretical weight: 12.58 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL34 Chain: Bg

Molecule details ›

Chain: Bg
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL29 Chain: Bh

Molecule details ›

Chain: Bh
Length: 123 amino acids
Theoretical weight: 14.57 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL36 Chain: Bi

Molecule details ›

Chain: Bi
Length: 105 amino acids
Theoretical weight: 12.26 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL37 Chain: Bj

Molecule details ›

Chain: Bj
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL38 Chain: Bk

Molecule details ›

Chain: Bk
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL39 Chain: Bl

Molecule details ›

Chain: Bl
Length: 51 amino acids
Theoretical weight: 6.46 KDa
Source organism: Oryctolagus cuniculus

60S ribosomal protein L40 Chain: Bm

Molecule details ›

Chain: Bm
Length: 128 amino acids
Theoretical weight: 14.8 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL42 Chain: Bo

Molecule details ›

Chain: Bo
Length: 106 amino acids
Theoretical weight: 12.49 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein eL43 Chain: Bp

Molecule details ›

Chain: Bp
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Oryctolagus cuniculus

Ribosomal protein eL28 Chain: Br

Molecule details ›

Chain: Br
Length: 137 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL10 Chain: Bs

Molecule details ›

Chain: Bs
Length: 318 amino acids
Theoretical weight: 34.38 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL11 Chain: Bt

Molecule details ›

Chain: Bt
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Oryctolagus cuniculus

Large ribosomal subunit protein uL1 Chain: Bv

Molecule details ›

Chain: Bv
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Oryctolagus cuniculus

Eukaryotic peptide chain release factor subunit 1 Chain: By

Molecule details ›

Chain: By
Length: 437 amino acids
Theoretical weight: 49.12 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21(DE3)

ATP binding cassette subfamily E member 1 Chain: Bz

Molecule details ›

Chain: Bz
Length: 599 amino acids
Theoretical weight: 67.41 KDa
Source organism: Oryctolagus cuniculus

18S Ribosomal RNA Chain: A2

Molecule details ›

Chain: A2
Length: 1870 nucleotides
Theoretical weight: 603.93 KDa
Source organism: Oryctolagus cuniculus
Sequence domains: Eukaryotic small subunit ribosomal RNA

mRNA containing SARS-CoV-2 sequence Chain: AH

Molecule details ›

Chain: AH
Length: 217 nucleotides
Theoretical weight: 69.68 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Not provided
Sequence domains: Coronavirus frameshifting stimulation element

E-site tRNA Chain: AI

Molecule details ›

Chain: AI
Length: 76 nucleotides
Theoretical weight: 15.21 KDa
Source organism: Bos taurus

P-site Leu-tRNA(Leu) Chain: AT

Molecule details ›

Chain: AT
Length: 86 nucleotides
Theoretical weight: 27.76 KDa
Source organism: Bos taurus
Sequence domains: tRNA

28S ribosomal RNA Chain: B5

Molecule details ›

Chain: B5
Length: 4808 nucleotides
Theoretical weight: 1557.52 KDa
Source organism: Oryctolagus cuniculus
Sequence domains: Eukaryotic large subunit ribosomal RNA

5S ribosomal RNA Chain: B7

Molecule details ›

Chain: B7
Length: 119 nucleotides
Theoretical weight: 38.35 KDa
Source organism: Oryctolagus cuniculus
Sequence domains: 5S ribosomal RNA

5.8S ribosomal RNA Chain: B8

Molecule details ›

Chain: B8
Length: 158 nucleotides
Theoretical weight: 50.81 KDa
Source organism: Oryctolagus cuniculus
Sequence domains: 5.8S ribosomal RNA