› 7nlc

X-ray diffraction
1.4Å resolution

Crystallographic structure of human Tsg101 UEV domain in complex with a HEV ORF3 peptide

Released:
DOI: 10.2210/pdb7nlc/pdb
PDB entry 7nlc coloured by chain, front view.
PDB entry 7nlc coloured by chain, side view.
PDB entry 7nlc coloured by chain, top view.
Source organisms:
Entry authors: Moschidi D, Dupre E, Villeret V, Hanoulle X

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189276 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor susceptibility gene 101 protein Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99816 (Residues: 1-145; Coverage: 37%)
Gene name: TSG101
Sequence domains: UEV domain
Protein ORF3 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 963 Da
Source organism: Orthohepevirus A
Expression system: Not provided
UniProt:
  • Canonical: Q9YLR0 (Residues: 93-102; Coverage: 9%)
Gene name: ORF3

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand SO4 1 x SO4
Ligand NH4 50 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 42.57Å b: 42.57Å c: 188.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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