› 7ll4

X-ray diffraction
1.31Å resolution

High-resolution crystal structure of human JAK2 kinase domain (JH1) bound to PN5-114.

Released:
DOI: 10.2210/pdb7ll4/pdb
PDB entry 7ll4 coloured by chain, front view.
PDB entry 7ll4 coloured by chain, side view.
PDB entry 7ll4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Karim MR, Schonbrunn E

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK2 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 34.94 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1
UniProt:
  • Canonical: O60674 (Residues: 839-1132; Coverage: 26%)
Gene name: JAK2
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

1 bound ligand:
Ligand Y5D 1 x Y5D
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 113.835Å b: 68.937Å c: 49.705Å
α: 90° β: 97.65° γ: 90°
R-values:
R R work R free
0.178 0.177 0.194
Expression system: Mammalian expression vector Flag-MCS-pcDNA3.1

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