› 7l0s

Electron Microscopy
4.5Å resolution

Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, with AHD

Released:
DOI: 10.2210/pdb7l0s/pdb
PDB entry 7l0s coloured by chain, front view.
PDB entry 7l0s coloured by chain, side view.
PDB entry 7l0s coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of an activated GPCR-G protein complex in lipid nanodiscs.
Zhang M, Gui M, Wang ZF, Gorgulla C, Yu JJ, Wu H, Sun ZJ, Klenk C, Merklinger L, Morstein L, Hagn F, Plückthun A, Brown A, Nasr ML, Wagner G
Nat Struct Mol Biol 28 258-267 (2021)
PMID: 33633398
Related structures: EMD-23102

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-148840 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Neurotensin receptor type 1 Chain: C
Molecule details ›
Chain: C
Length: 336 amino acids
Theoretical weight: 37.48 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20789 (Residues: 50-390; Coverage: 76%)
Gene names: Ntsr, Ntsr1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Neurotensin Chain: D
Molecule details ›
Chain: D
Length: 10 amino acids
Theoretical weight: 1.09 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20068 (Residues: 157-162; Coverage: 4%)
Gene name: Nts
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 361 amino acids
Theoretical weight: 39.98 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(T) subunit gamma-T1 Chain: G
Molecule details ›
Chain: G
Length: 83 amino acids
Theoretical weight: 9.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63211 (Residues: 2-74; Coverage: 99%)
Gene name: GNGT1
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-23102
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

17 review citations

Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches.
Jones et al. (2020)
16 more

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more