› 7jsn

Electron Microscopy
3.2Å resolution

Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6

Released:
DOI: 10.2210/pdb7jsn/pdb
PDB entry 7jsn coloured by chain, front view.
PDB entry 7jsn coloured by chain, side view.
PDB entry 7jsn coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6.
Gao Y, Eskici G, Ramachandran S, Poitevin F, Seven AB, Panova O, Skiniotis G, Cerione RA
Mol Cell 80 237-245.e4 (2020)
PMID: 33007200
Related structures: EMD-22458

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-138154 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 859 amino acids
Theoretical weight: 99.46 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P11541 (Residues: 1-859; Coverage: 100%)
Gene names: PDE6A, PDEA
Sequence domains:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 853 amino acids
Theoretical weight: 98.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P23439 (Residues: 1-853; Coverage: 100%)
Gene names: PDE6B, PDEB
Sequence domains:
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 371 amino acids
Theoretical weight: 42.22 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04695 (Residues: 1-350; Coverage: 100%)
Gene name: GNAT1
Sequence domains: G-protein alpha subunit
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma Chains: C, D
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 9.68 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P04972 (Residues: 1-87; Coverage: 100%)
Gene names: PDE6G, PDEG
Sequence domains: Retinal cGMP phosphodiesterase, gamma subunit

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand VDN 2 x VDN
Ligand 35G 2 x 35G
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-22458
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New focus on regulation of the rod photoreceptor phosphodiesterase.
Gulati et al. (2021)
1 more

10 mentions without citation

Photoreceptor phosphodiesterase (PDE6): activation and inactivation mechanisms during visual transduction in rods and cones.
Cote RH. (2021)
9 more