› 7fih

Electron Microscopy
3.2Å resolution

luteinizing hormone/choriogonadotropin receptor(S277I)-chorionic gonadotropin-Gs-Org43553 complex

Released:
DOI: 10.2210/pdb7fih/pdb
PDB entry 7fih coloured by chain, front view.
PDB entry 7fih coloured by chain, side view.
PDB entry 7fih coloured by chain, top view.
Source organisms:
Primary publication:
Structures of full-length glycoprotein hormone receptor signalling complexes.
Duan J, Xu P, Cheng X, Mao C, Croll T, He X, Shi J, Luan X, Yin W, You E, Liu Q, Zhang S, Jiang H, Zhang Y, Jiang Y, Xu HE
Nature 598 688-692 (2021)
PMID: 34552239
Related structures: EMD-31597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275985 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Engineered Guanine nucleotide-binding protein G(s) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.88 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.61 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Lutropin-choriogonadotropic hormone receptor Chain: R
Molecule details ›
Chain: R
Length: 697 amino acids
Theoretical weight: 78.66 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P22888 (Residues: 28-699; Coverage: 100%)
Gene names: LCGR, LGR2, LHCGR, LHRHR
Sequence domains:
Glycoprotein hormones alpha chain Chain: X
Molecule details ›
Chain: X
Length: 115 amino acids
Theoretical weight: 12.96 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 2-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Choriogonadotropin subunit beta 3 Chain: Y
Molecule details ›
Chain: Y
Length: 165 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DN86 (Residues: 1-165; Coverage: 100%)
Gene names: CGB, CGB3, CGB5, CGB8
Sequence domains: Cystine-knot domain

Ligands and Environments

1 bound ligand:
Ligand 55Z 1 x 55Z
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-31597
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

8 review citations

Allosteric modulation of G protein-coupled receptor signaling.
Shen et al. (2023)
7 more

7 mentions without citation

AlphaFold2 versus experimental structures: evaluation on G protein-coupled receptors.
He et al. (2023)
6 more