› 7f2o

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the type 2 bradykinin receptor in complex with the bradykinin and an Gq protein

Released:
DOI: 10.2210/pdb7f2o/pdb
PDB entry 7f2o coloured by chain, front view.
PDB entry 7f2o coloured by chain, side view.
PDB entry 7f2o coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for kinin selectivity and activation of the human bradykinin receptors.
Yin YL, Ye C, Zhou F, Wang J, Yang D, Yin W, Wang MW, Xu HE, Jiang Y
Nat Struct Mol Biol 28 755-761 (2021)
PMID: 34518695
Related structures: EMD-31429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-279412 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
G subunit q (Gi1-Gq chimeric) Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 377 amino acids
Theoretical weight: 41.06 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Bradykinin Chain: D
Molecule details ›
Chain: D
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01042 (Residues: 381-389; Coverage: 1%)
Gene names: BDK, KNG, KNG1
B2 bradykinin receptor Chain: R
Molecule details ›
Chain: R
Length: 595 amino acids
Theoretical weight: 67.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30411 (Residues: 40-370; Coverage: 85%)
Gene names: BDKRB2, BKR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
single Fab chain (svFv16) Chain: S
Molecule details ›
Chain: S
Length: 248 amino acids
Theoretical weight: 26.39 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-31429
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

5 review citations

Peptidome: Chaos or Inevitability.
Lyapina et al. (2021)
4 more

6 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
5 more