› 7dn6

X-ray diffraction
1.7Å resolution

Crystal structure of bovine lactoperoxidase with hydrogen peroxide trapped between heme iron and his109 at 1.69 A resolution

Released:
DOI: 10.2210/pdb7dn6/pdb
PDB entry 7dn6 coloured by chain, front view.
PDB entry 7dn6 coloured by chain, side view.
PDB entry 7dn6 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of a ternary complex of lactoperoxidase with iodide and hydrogen peroxide at 1.77 Å resolution.
Singh PK, Sharma P, Bhushan A, Kaur P, Sharma S, Singh TP
J Inorg Biochem 220 111461 (2021)
PMID: 33882424

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lactoperoxidase Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.77 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80025 (Residues: 118-712; Coverage: 86%)
Gene name: LPO
Sequence domains: Animal haem peroxidase

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
8 bound ligands:
Ligand CA 1 x CA
Ligand NAG 2 x NAG
Ligand IOD 19 x IOD
Ligand GOL 1 x GOL
Ligand EDO 1 x EDO
Ligand ZN 1 x ZN
Ligand OSM 3 x OSM
Ligand PEO 1 x PEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 53.913Å b: 79.69Å c: 76.965Å
α: 90° β: 102.23° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235

Quick links

Citations

4 citation in other articles

In Situ Structural Observation of a Substrate- and Peroxide-Bound High-Spin Ferric-Hydroperoxo Intermediate in the P450 Enzyme CYP121.
Nguyen et al. (2023)
3 more

1 mention without citation

Cryotrapping peroxide in the active site of human mitochondrial manganese superoxide dismutase crystals for neutron diffraction.
Azadmanesh et al. (2022)