› 7db6

Electron Microscopy
3.3Å resolution

human melatonin receptor MT1 - Gi1 complex

Released:
DOI: 10.2210/pdb7db6/pdb
PDB entry 7db6 coloured by chain, front view.
PDB entry 7db6 coloured by chain, side view.
PDB entry 7db6 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the human MT1-Gi signaling complex.
Okamoto HH, Miyauchi H, Inoue A, Raimondi F, Tsujimoto H, Kusakizako T, Shihoya W, Yamashita K, Suno R, Nomura N, Kobayashi T, Iwata S, Nishizawa T, Nureki O
Nat Struct Mol Biol 28 694-701 (2021)
PMID: 34354246
Related structures: EMD-30627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211268 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 344 amino acids
Theoretical weight: 37.8 KDa
Source organism: Rattus rattus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 260 amino acids
Theoretical weight: 27.72 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Melatonin receptor type 1A Chain: D
Molecule details ›
Chain: D
Length: 372 amino acids
Theoretical weight: 42.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P48039 (Residues: 1-340; Coverage: 97%)
Gene name: MTNR1A
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand JEV 1 x JEV
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-30627
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

2 review citations

Structural basis of GPCR coupling to distinct signal transducers: implications for biased signaling.
Seyedabadi et al. (2022)
1 more

6 mentions without citation

AlphaFold2 versus experimental structures: evaluation on G protein-coupled receptors.
He et al. (2023)
5 more