7d68

Electron Microscopy
3Å resolution

Cryo-EM structure of the human glucagon-like peptide-2 receptor-Gs protein complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208188 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.9 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63097 (Residues: 1-3, 61-184; Coverage: 36%)
  • Canonical: P04896 (Residues: 4-67, 208-394; Coverage: 64%)
Gene names: GNAI1, GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Not provided
Glucagon-like peptide 2 Chain: P
Molecule details ›
Chain: P
Length: 33 amino acids
Theoretical weight: 3.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01275 (Residues: 146-178; Coverage: 21%)
Gene name: GCG
Sequence domains: Peptide hormone
Glucagon-like peptide 2 receptor Chain: R
Molecule details ›
Chain: R
Length: 664 amino acids
Theoretical weight: 75.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O95838 (Residues: 1-490; Coverage: 89%)
Gene name: GLP2R
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-30590
Expression systems:
  • Spodoptera frugiperda
  • Not provided