› 7cz5

Electron Microscopy
2.6Å resolution

Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex

Released:
DOI: 10.2210/pdb7cz5/pdb
PDB entry 7cz5 coloured by chain, front view.
PDB entry 7cz5 coloured by chain, side view.
PDB entry 7cz5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for activation of the growth hormone-releasing hormone receptor.
Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun 11 5205 (2020)
PMID: 33060564
Related structures: EMD-30505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208390 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Growth hormone-releasing hormone receptor Chain: R
Molecule details ›
Chain: R
Length: 556 amino acids
Theoretical weight: 61.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02643 (Residues: 23-405; Coverage: 96%)
Gene name: GHRHR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Somatoliberin Chain: P
Molecule details ›
Chain: P
Length: 44 amino acids
Theoretical weight: 5.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01286 (Residues: 32-75; Coverage: 50%)
Gene names: GHRF, GHRH
Sequence domains: Peptide hormone
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 400 amino acids
Theoretical weight: 43.71 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand PLM 2 x PLM
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-30505
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli

Quick links

Citations

2 review citations

Prolactin and Growth Hormone Signaling and Interlink Focused on the Mammosomatotroph Paradigm: A Comprehensive Review of the Literature.
Araujo-Castro et al. (2023)
1 more

5 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
4 more