7cro

Electron Microscopy
3.75Å resolution

NSD2 bearing E1099K/T1150A dual mutation in complex with 187-bp NCP

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(36) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(36)
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3 Chains: E, M
Molecule details ›
Chains: E, M
Length: 135 amino acids
Theoretical weight: 15.22 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92133 (Residues: 2-136; Coverage: 99%)
Gene names: H3l, h3c8, h3c8.S, hist1h3g
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Sequence domains:
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 122 amino acids
Theoretical weight: 13.52 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Histone-lysine N-methyltransferase NSD2 Chain: I
Molecule details ›
Chain: I
Length: 705 amino acids
Theoretical weight: 79.69 KDa
Source organism: Homo sapiens
Expression system: Baculovirus transfer vector pFASTBAC1
UniProt:
  • Canonical: O96028 (Residues: 661-1365; Coverage: 52%)
Gene names: KIAA1090, MMSET, NSD2, TRX5, WHSC1
Sequence domains:
DNA (168-MER) Chain: A
Molecule details ›
Chain: A
Length: 187 nucleotides
Theoretical weight: 57.49 KDa
DNA (168-MER) Chain: K
Molecule details ›
Chain: K
Length: 187 nucleotides
Theoretical weight: 57.98 KDa

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.75Å
Relevant EMDB volumes: EMD-30453
Expression systems:
  • Escherichia coli
  • Baculovirus transfer vector pFASTBAC1