› 7bh0

X-ray diffraction
2.1Å resolution

Mutant L39Y of recombinant beta-lactoglobulin in complex with endogenous ligand

Released:
DOI: 10.2210/pdb7bh0/pdb
PDB entry 7bh0 coloured by chain, front view.
PDB entry 7bh0 coloured by chain, side view.
PDB entry 7bh0 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Interactions of new lactoglobulin variants with tetracaine: crystallographic studies of ligand binding to lactoglobulin mutants possessing single substitution in the binding pocket.
Loch J, Bonarek P, Siuda M, Wróbel P, Lewiński K
Acta Biochim Pol 68 23-28 (2021)
PMID: 33719368

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: AAA
Molecule details ›
Chain: AAA
Length: 162 amino acids
Theoretical weight: 18.28 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family

Ligands and Environments

2 bound ligands:
Ligand DAO 1 x DAO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P3221
Unit cell:
a: 53.27Å b: 53.27Å c: 109.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.201 0.275
Expression system: Escherichia coli BL21

Quick links

Citations

1 citations in other articles

New ligand-binding sites identified in the crystal structures of β-lactoglobulin complexes with desipramine.
Loch et al. (2022)