› 7ajf

Electron Microscopy
8.45Å resolution

bovine ATP synthase dimer state2:state2

Released:
DOI: 10.2210/pdb7ajf/pdb
PDB entry 7ajf coloured by chain, front view.
PDB entry 7ajf coloured by chain, side view.
PDB entry 7ajf coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Interface mobility between monomers in dimeric bovine ATP synthase participates in the ultrastructure of inner mitochondrial membranes.
Spikes TE, Montgomery MG, Walker JE
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33542155
Related structures: EMD-11432

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-133626 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, AA, AB, AC, B, C
Molecule details ›
Chains: A, AA, AB, AC, B, C
Length: 510 amino acids
Theoretical weight: 55.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P19483 (Residues: 44-553; Coverage: 92%)
Gene names: ATP5A1, ATP5A2, ATP5F1A
Sequence domains:
ATP synthase subunit beta, mitochondrial Chains: AD, AE, AF, D, E, F
Molecule details ›
Chains: AD, AE, AF, D, E, F
Length: 482 amino acids
Theoretical weight: 51.76 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 47-528; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
ATP synthase subunit gamma, mitochondrial Chains: AG, G
Molecule details ›
Chains: AG, G
Length: 273 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-298; Coverage: 92%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase
ATP synthase subunit delta, mitochondrial Chains: AH, H
Molecule details ›
Chains: AH, H
Length: 146 amino acids
Theoretical weight: 15.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05630 (Residues: 23-168; Coverage: 87%)
Gene names: ATP5D, ATP5F1D
Sequence domains:
ATP synthase subunit epsilon, mitochondrial Chains: AI, I
Molecule details ›
Chains: AI, I
Length: 50 amino acids
Theoretical weight: 5.66 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05632 (Residues: 2-51; Coverage: 98%)
Gene names: ATP5E, ATP5F1E
Sequence domains: Mitochondrial ATP synthase epsilon chain
ATPase inhibitor, mitochondrial Chains: AJ, J
Molecule details ›
Chains: AJ, J
Length: 84 amino acids
Theoretical weight: 9.6 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01096 (Residues: 26-109; Coverage: 77%)
Gene names: ATP5IF1, ATPI, ATPIF1
Sequence domains: Mitochondrial ATPase inhibitor, IATP
ATP synthase F(0) complex subunit C2, mitochondrial Chains: AK, AL, AM, AN, AO, AP, AQ, AR, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: AK, AL, AM, AN, AO, AP, AQ, AR, K, L, M, N, O, P, Q, R
Length: 75 amino acids
Theoretical weight: 7.65 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07926 (Residues: 69-143; Coverage: 52%)
Gene names: ATP5G2, ATP5MC2
Sequence domains: ATP synthase subunit C
ATP synthase subunit O, mitochondrial Chains: AS, S
Molecule details ›
Chains: AS, S
Length: 190 amino acids
Theoretical weight: 20.96 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13621 (Residues: 24-213; Coverage: 89%)
Gene names: ATP5O, ATP5PO
Sequence domains: ATP synthase delta (OSCP) subunit
ATP synthase subunit a Chains: Aa, a
Molecule details ›
Chains: Aa, a
Length: 226 amino acids
Theoretical weight: 24.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00847 (Residues: 1-226; Coverage: 100%)
Gene names: ATP6, ATPASE6, MT-ATP6, MTATP6
Sequence domains: ATP synthase A chain
ATP synthase F(0) complex subunit B1, mitochondrial Chains: Ab, b
Molecule details ›
Chains: Ab, b
Length: 214 amino acids
Theoretical weight: 24.7 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13619 (Residues: 43-256; Coverage: 84%)
Gene names: ATP5F, ATP5F1, ATP5PB
Sequence domains: Mitochondrial ATP synthase B chain precursor (ATP-synt_B)
ATP synthase subunit d, mitochondrial Chains: Ad, d
Molecule details ›
Chains: Ad, d
Length: 160 amino acids
Theoretical weight: 18.59 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13620 (Residues: 2-161; Coverage: 99%)
Gene names: ATP5H, ATP5PD
Sequence domains: ATP synthase D chain, mitochondrial (ATP5H)
ATP synthase subunit e, mitochondrial Chains: Ae, e
Molecule details ›
Chains: Ae, e
Length: 70 amino acids
Theoretical weight: 8.21 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q00361 (Residues: 2-71; Coverage: 99%)
Gene names: ATP5I, ATP5ME
Sequence domains: ATP synthase E chain
ATP synthase subunit f, mitochondrial Chains: Af, f
Molecule details ›
Chains: Af, f
Length: 87 amino acids
Theoretical weight: 10.18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q28851 (Residues: 2-88; Coverage: 99%)
Gene names: ATP5J2, ATP5MF
Sequence domains: Mitochondrial F1F0-ATP synthase, subunit f
ATP synthase subunit g, mitochondrial Chains: Ag, g
Molecule details ›
Chains: Ag, g
Length: 102 amino acids
Theoretical weight: 11.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q28852 (Residues: 2-103; Coverage: 99%)
Gene names: ATP5L, ATP5MG
Sequence domains: Mitochondrial ATP synthase g subunit
ATP synthase-coupling factor 6, mitochondrial Chains: Ah, h
Molecule details ›
Chains: Ah, h
Length: 76 amino acids
Theoretical weight: 8.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02721 (Residues: 33-108; Coverage: 70%)
Gene names: ATP5J, ATP5PF
Sequence domains: Mitochondrial ATP synthase coupling factor 6
ATP synthase subunit ATP5MJ, mitochondrial Chains: Aj, j
Molecule details ›
Chains: Aj, j
Length: 60 amino acids
Theoretical weight: 6.85 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P14790 (Residues: 1-60; Coverage: 100%)
Gene names: ATP5MJ, ATP5MPL, MP68
Sequence domains: Mitochondrial proteolipid
ATP synthase membrane subunit K, mitochondrial Chains: Ak, k
Molecule details ›
Chains: Ak, k
Length: 57 amino acids
Theoretical weight: 6.31 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZBI7 (Residues: 2-58; Coverage: 98%)
Gene names: ATP5MK, ATPMD, DAPIT, USMG5
Sequence domains: ATP synthase regulation
ATP synthase protein 8 Chains: 8, A8
Molecule details ›
Chains: 8, A8
Length: 66 amino acids
Theoretical weight: 7.94 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P03929 (Residues: 1-66; Coverage: 100%)
Gene names: ATP8, ATPASE8, MT-ATP8, MTATP8
Sequence domains: ATP synthase protein 8

Ligands and Environments

2 bound ligands:
Ligand CDL 6 x CDL
Ligand LHG 4 x LHG
1 modified residue:
Ligand M3L 16 x M3L

Experiments and Validation Details

Entry percentile scores
Resolution: 8.45Å
Relevant EMDB volumes: EMD-11432
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The mitochondrial permeability transition: Recent progress and open questions.
Bernardi et al. (2022)
5 more