› 6zyl

X-ray diffraction
2.09Å resolution

non-heme monooxygenase; ThoJ apo

Released:
DOI: 10.2210/pdb6zyl/pdb
PDB 6zyl coloured by chain and viewed from the front.
PDB 6zyl coloured by chain and viewed from the side.
PDB 6zyl coloured by chain and viewed from the top.
Source organism: Streptomyces malaysiense
Primary publication:
Non-Heme Monooxygenase ThoJ Catalyzes Thioholgamide β-Hydroxylation.
Sikandar A, Lopatniuk M, Luzhetskyy A, Koehnke J
ACS Chem Biol 15 2815-2819 (2020)
PMID: 32965102

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 33.58 KDa
Source organism: Streptomyces malaysiense
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1J4PXK4 (Residues: 1-290; Coverage: 100%)
Gene name: VT52_024865
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

1 bound ligand:
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 80.517Å b: 80.517Å c: 130.144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.207
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 citations in other articles

Understanding thioamitide biosynthesis using pathway engineering and untargeted metabolomics.
Eyles et al. (2021)