› 6zvo

X-ray diffraction
1.37Å resolution

Crystal structure of unliganded MLKL executioner domain

Released:
DOI: 10.2210/pdb6zvo/pdb
PDB entry 6zvo coloured by chain, front view.
PDB entry 6zvo coloured by chain, side view.
PDB entry 6zvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Locking mixed-lineage kinase domain-like protein in its auto-inhibited state prevents necroptosis.
Rübbelke M, Fiegen D, Bauer M, Binder F, Hamilton J, King J, Thamm S, Nar H, Zeeb M
Proc Natl Acad Sci U S A 117 33272-33281 (2020)
PMID: 33318170

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mixed lineage kinase domain-like protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 17.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NB16 (Residues: 2-150; Coverage: 32%)
Gene name: MLKL

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand BR 1 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 56.057Å b: 53.501Å c: 70.912Å
α: 90° β: 98.36° γ: 90°
R-values:
R R work R free
0.192 0.19 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

MLKL in cancer: more than a necroptosis regulator.
Martens et al. (2021)
5 more

3 mentions without citation

From (Tool)Bench to Bedside: The Potential of Necroptosis Inhibitors.
Gardner et al. (2023)
2 more