› 6zti

X-ray diffraction
1.81Å resolution

Phospholipase PlaB from Legionella pneumophila in complex with thio-NAD

Released:
DOI: 10.2210/pdb6zti/pdb
PDB 6zti coloured by chain and viewed from the front.
PDB 6zti coloured by chain and viewed from the side.
PDB 6zti coloured by chain and viewed from the top.
Source organism: Legionella pneumophila
Primary publication:
NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB.
Diwo M, Michel W, Aurass P, Kuhle-Keindorf K, Pippel J, Krausze J, Wamp S, Lang C, Blankenfeldt W, Flieger A
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34074754

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PlaB phospholipase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 489 amino acids
Theoretical weight: 55.39 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A378K488 (Residues: 1-474; Coverage: 100%)
Gene names: NCTC12000_01733, plaB

Ligands and Environments

2 bound ligands:
Ligand SND 8 x SND
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 75.831Å b: 90.725Å c: 92.79Å
α: 78.44° β: 90.26° γ: 75.41°
R-values:
R R work R free
0.188 0.186 0.234
Expression system: Escherichia coli

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