› 6zsm

X-ray diffraction
1.95Å resolution

Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)

Released:
DOI: 10.2210/pdb6zsm/pdb
PDB 6zsm coloured by chain and viewed from the front.
PDB 6zsm coloured by chain and viewed from the side.
PDB 6zsm coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.
Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC
Nat Biotechnol (2021)
PMID: 34845372

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; RCaMP Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.32 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 51-59, 60-94, 95-127, 128-147, 218-218, 225-269, 295-300, 312-336, 348-362, 369-432; Coverage: 56%)
  • Canonical: P42212 (Residues: 2-78, 80-87, 133-159, 166-176, 183-193, 209-214; Coverage: 56%)
Gene name: GFP

Ligands and Environments

3 bound ligands:
Ligand CA 5 x CA
Ligand FMT 14 x FMT
Ligand EDO 5 x EDO
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 121.54Å b: 121.54Å c: 96.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.198
Expression system: Escherichia coli

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