6zsm

X-ray diffraction
1.95Å resolution

Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; RCaMP Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.32 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 51-59, 60-94, 95-127, 128-147, 218-218, 225-269, 295-300, 312-336, 348-362, 369-432; Coverage: 56%)
  • Canonical: P42212 (Residues: 2-78, 80-87, 133-159, 166-176, 183-193, 209-214; Coverage: 56%)
Gene name: GFP

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P41212
Unit cell:
a: 121.54Å b: 121.54Å c: 96.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.198
Expression system: Escherichia coli