› 6zs0

X-ray diffraction
1.5Å resolution

Crystal structure of 5-dimethylallyltryptophan synthase from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb6zs0/pdb
PDB entry 6zs0 coloured by chain, front view.
PDB entry 6zs0 coloured by chain, side view.
PDB entry 6zs0 coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Reprogramming Substrate and Catalytic Promiscuity of Tryptophan Prenyltransferases.
Ostertag E, Zheng L, Broger K, Stehle T, Li SM, Zocher G
J Mol Biol 433 166726 (2021)
PMID: 33249189

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DMATS type aromatic prenyltransferase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 370 amino acids
Theoretical weight: 39.68 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 44.88Å b: 70.454Å c: 91.145Å
α: 90° β: 92.946° γ: 90°
R-values:
R R work R free
0.19 0.189 0.22
Expression system: Escherichia coli

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Citations

2 review citations

Redesigning Enzymes for Biocatalysis: Exploiting Structural Understanding for Improved Selectivity.
Ding et al. (2022)
1 more