› 6zpb

X-ray diffraction
1.72Å resolution

Cyanophage S-2L HD phosphohydrolase (DatZ) bound to dA and two catalytic Co2+ ions

Released:
DOI: 10.2210/pdb6zpb/pdb
PDB entry 6zpb coloured by chain, front view.
PDB entry 6zpb coloured by chain, side view.
PDB entry 6zpb coloured by chain, top view.
Source organism: Cyanophage S-2L
Primary publication:
How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA.
Czernecki D, Legrand P, Tekpinar M, Rosario S, Kaminski PA, Delarue M
OpenAccess logo Nat Commun 12 2420 (2021)
PMID: 33893297

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DatZ Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.61 KDa
Source organism: Cyanophage S-2L
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand 3D1 1 x 3D1
Ligand CO 3 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: R32
Unit cell:
a: 141.827Å b: 141.827Å c: 53.725Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.146 0.17
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacteriophage-Encoded DNA Polymerases-Beyond the Traditional View of Polymerase Activities.
Morcinek-Orłowska et al. (2022)
1 more

1 mention without citation

How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA.
Czernecki et al. (2021)